Cytochrome P450 monooxygenase (CYP)

Tsananguro pfupi:

NezveCYP

ES-CYPs: Rudzi rwema enzymes e redox ane mercaptan-heme senzvimbo inoshanda yekufambisa okisijeni. Aya ndiwo ma terminal oxygenases uye anoda heme kuti aite kuti okisijeni ishande.

Cytochrome P450 monooxygenase inokonzeresa oxidation, epoxidation uye hydroxylation yealkyl, hydroxylation uye oxidation yeammonia, oxidation yesarufa, dealkylation (oxygen, sarufa, ammonia), oxidative dehydrogenation, deamination uye dehalogenation.
Kune mhando sere dzezvigadzirwa zve cytochrome P450 monooxygenase (Nhamba seES-CYP-101～ES-CYP-108) zvakagadzirwa neSyncoZymes.

Nharembozha/Wechat/WhatsApp: +86-13681683526

Tsamba yemagetsi:lchen@syncozymes.com

Tumira email kwatiri

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

NezveCYP:

Rudzi rwekuita kweCatalytic:

Ruzivo rweChigadzirwa:

MaEnzayimu	Kiti Yekuongorora (SynKit)	Tsanangudzo
Upfu hweEnzimu	ES-CYP-101~ ES-CYP-108	seti ye8 Cytochrome P450 Monooxygenases, 50 mg chimwe nechimwe zvinhu 8 * 50mg / chinhu, kana chimwe chiyero
Kiti Yekuongorora (SynKit)	ES-CYP-800	seti ye8 Cytochrome P450 Monooxygenases, 1mg chimwe nechimwe zvinhu 8 * 1mg / chinhu

Zvakanakira:

★ Broad substrate spectrum.
★ Kuchinja kwakanyanya.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.

Mirayiridzo yekushandisa:

➢ Kazhinji, nzira yekuita nayo zvinhu inofanira kusanganisira substrate, buffer solution (Iyo yakanakisisa pH yeenzyme), coenzyme (NAD(H) kana NADP(H)), coenzyme regeneration system (semuenzaniso glucose neglucose dehydrogenase) uye ES-CYP. coenzyme necoenzyme regeneration system zvinogona kutsiviwa nehydrogen peroxide muchikamu che reaction system.
➢ Mhando dzese dzeES-CYPs dzinoenderana nemamiriro akasiyana-siyana ekuita zvakanaka dzinofanira kudzidzwa imwe neimwe.
➢ Substrate yakawanda kana chigadzirwa chine substrate chinogona kukanganisa mashandiro eES-CYP. Zvisinei, kudziviswa kunogona kuderedzwa nekuwedzera substrate.

Mienzaniso yeMashandisirwo:

Muenzaniso 1⁽¹⁾:

Muenzaniso 2⁽²⁾:

Muenzaniso 3⁽³⁾:

Muenzaniso 4⁽⁴⁾:

Kuchengetera:

Chengeta kwemakore maviri pasi pe -20℃.

Kuchenjerera:

Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent yakakwira.

Mareferensi:

1. Zaretzki J, Matlock M, naSwamidass S JJ Chem. Inf. Model, 2013, 53, 3373–3383.
2. Gannett P M., Kabulski J, Perez F A., e tal. J. Am. Chem. Soc., 2006, 128 (26), 8374–8375.
3. Cryle M J., Matovic N J., naDe Voss J J. Org. Lett., 2003, 5 (18), 3341–3344.
4. Kawauchi, H., Sasaki, J., Adachi, T., e tal. Biochim. Biophys. Acta, 1994, 1219, 179.
5. Yasutake, Y., Fujii, Y.; Cheon, WK e tal. Acta Crystallog. 2009, 65, 372.