Transaminase (ATA)
Enzymes: Ari macromolecular biological catalysts, ma enzymes mazhinji mapuroteni.
Transaminases: Kirasi yema enzymes anokonzera kutamisa amino pakati peamino acids uye keto acids.Transaminases ndiwo akakosha biological enzymes mune asymmetric synthesis uye racemic resolution ye chiral amines.
Aminotransferase inogona kukamurwa kuita makirasi mana zvichienderana nekutevedzana uye chimiro: Ⅰ, Ⅱ, Ⅲ uye Ⅳ.ω-aminotransferases ndeyekirasi II transaminases, inowanzoshandiswa mukugadzirwa kwechiral amines uye asiri ega amino acids, akadai se β-amino acids.
ω-aminotransferases: Kazhinji, ω-transaminase inoreva kirasi yema enzymes, iyo catalytic ammonia kutamisa maitiro pasina α-amino acid se substrate kana chigadzirwa.
Catalytic mechanism:
| Enzymes | Product Code | Product Code |
| Enzyme Powder | ES-ATA-101~ ES-ATA-165 | seti ye65 ω-Transaminases, 50 mg imwe neimwe 65 zvinhu * 50mg / chinhu, kana humwe huwandu |
| Screening Kit (SynKit) | ES-ATA-6500 | seti ye65 ω-Transaminases, 1 MG chimwe nechimwe 65 zvinhu * 1mg / chinhu |
★ High substrate chaiyo.
★ Strong chiral selectivity.
★ High kutendeuka kushanda zvakanaka.
★ Zvishoma ne-zvigadzirwa.
★ Mild reaction conditions.
★ Kuchengetedza zvakatipoteredza.
➢ Kuongororwa kwe enzyme kunofanirwa kuitirwa kune chaiwo ma substrate nekuda kweiyo substrate chaiyo, uye kuwana enzyme inokonzeresa iyo yakanangwa substrate ine yakanakisa catalytic mhedzisiro.
➢ Usambofa wakabata nemamiriro akanyanya akadai se: tembiricha yepamusoro, yakakwirira/yakaderera pH uye organic solvent ine yakanyanya kugadzikana.
➢ Kazhinji, maitiro ekugadzirisa anofanirwa kusanganisira substrate, buffer solution, amino donor (yakadai seamino acids uye 1-phenyl ethylamine) kana receptor (yakadai seketo acids), coenzyme (PLP), cosolvent (yakadai seDMSO).
➢ ATA inofanirwa kuwedzerwa kwekupedzisira mukuita system, mushure mekunge pH uye tembiricha zvagadziriswa kune mamiriro ekuita.
➢ Ese marudzi eATA ane akasiyana optimum reaction mamiriro, saka imwe neimwe yadzo inofanirwa kuenderera mberi ichidzidzwa yega.
Muenzaniso 1(synthesis yeSitagliptin, asymmetric synthesis)(1):
Muenzaniso 2 (Mexiletine, Musanganiswa we kinetic resolution ine asymmetric synthesis)(2):
1 Savile CK, Janey JM, Mundorff EC, nevamwe.Sayenzi, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, nevamwe.Organic tsamba, 2009,11(21):4810-4812.
