Ene reductase (ERED)
ES-EREDs inokonzeresa mhando dzakasiyana dze substrate nekuda kwehupamhi hwema substrates.Kazhinji, C = C ye α, β -unsaturated compounds nemapoka e-electron-absorbing (kusanganisira ketone aldehyde, mapoka e nitro, carboxylic acids, esters, anhydride, lactones, imines, nezvimwewo) zviri nyore kuderedzwa ne ES-EREDs, asi the unactivated double bonds kwete.
Kune makumi mana nematanhatu emhando dzeERED enzyme zvigadzirwa (Nhamba seES-ERED-101~ES-ERED-146) yakagadzirwa neSyncoZymes.
Catalytic mechanism:
| Enzymes | Product Code | Tsanangudzo |
| Enzyme Powder | ES-ERED-101~ ES-ERED-146 | seti ye46 Ene Reductases, 50 mg imwe neimwe 46 zvinhu * 50mg / chinhu, kana humwe huwandu |
| Screening Kit (SynKit) | ES-ERED-4600 | seti ye46 Ene Reductases, 50 mg imwe neimwe 46 zvinhu * 50mg / chinhu, kana humwe huwandu |
★ High substrate chaiyo.
★ Strong chiral selectivity.
★ Kutendeuka kwepamusoro.
★ Zvishoma ne-zvigadzirwa.
★ Mild reaction conditions.
★ Kuchengetedza zvakatipoteredza.
★ Kuchengetedzwa kwepamusoro.
➢ Kazhinji, maitiro ekugadzirisa anofanirwa kusanganisira substrate, buffer solution (optimum reaction pH), coenzymes(NAD(H) kana NADP (H)), coenzyme regeneration system (eg glucose uye glucose dehydrogenase) uye ES-ERED.
➢ Ese maES-ERED anogona kuyedzwa zvakateerana muhurongwa hwekuita pamusoro kana neERED Screening Kit (SynKit ERED).
➢ Mhando dzese dzeES-ERED dzinoenderana neakasiyana optimum reaction mamiriro anofanirwa kudzidzwa ega.
➢ High concentration yeSubstrate kana chigadzirwa chine chinogona kutadzisa ES-ERED kuita.Nekudaro, iyo inhibition inogona kudzikiswa nebatch kuwedzera kwe substrate.
Muenzaniso 1(α,β-unsaturated aldehydes kana ketoni)(1):
Muenzaniso 2(α,β-unsaturated carboxylic acids uye zvinobva kwavari)(2):
Chengeta makore maviri pasi -20 ℃.
Usambofa wakasangana nemamiriro akanyanya akadai se: tembiricha yakakwira, yakakwira / yakaderera pH uye yakakwira concentration organic solvent.
1. Lucídio C, Fardelone J, Augusto R, e tal.J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, e tal..Org.Lett, 2007, 9(26): 5409-5411.
