Ene reductase (ERED)
MaES-ERED anokonzeresa mhando dzakasiyana dzesubstrate nekuda kwemhando dzakasiyana dzesubstrate. Kazhinji, C=C yemakomikoro eα, β-unsaturated ane ma electron-absorbing groups (kusanganisira ketone aldehyde, nitro groups, carboxylic acids, esters, anhydride, lactones, imines, nezvimwewo) anoderedzwa zviri nyore neES-EREDs, asi ma double bonds asina kushanda haadaro.
Kune mhando makumi mana nematanhatu dzezvigadzirwa zve enzyme yeERED (Nhamba seES-ERED-101~ES-ERED-146) yakagadzirwa neSyncoZymes.
Maitiro ekugadzirisa:
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-ERED-101~ ES-ERED-146 | seti ye46 Ene Reductases, 50 mg chimwe nechimwe 46 zvinhu * 50mg / chinhu, kana chimwe chiyero |
| Kiti Yekuongorora (SynKit) | ES-ERED-4600 | seti ye46 Ene Reductases, 50 mg chimwe nechimwe 46 zvinhu * 50mg / chinhu, kana chimwe chiyero |
★ Hunhu hwepamusoro hwe substrate.
★ Kusarudza kwakasimba kwerudo rwechikara.
★ Kuchinja kwakanyanya.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.
★ Kuchengetedzwa kwakanyanya.
➢ Kazhinji, nzira yekugadzirira inofanira kusanganisira substrate, buffer solution (optimum reaction pH), coenzymes(NAD(H) kana NADP(H)), coenzyme regeneration system (semuenzaniso glucose neglucose dehydrogenase) uye ES-ERED.
➢ MaES-ERED ese anogona kuyedzwa zvakateerana mu reaction system iri pamusoro apa kana neERED Screening Kit (SynKit ERED).
➢ Mhando dzese dzeES-EREDs dzinoenderana nemamiriro akasiyana-siyana ekuita zvakanaka dzinofanira kudzidzwa imwe neimwe.
➢ Substrate yakawanda kana chigadzirwa chine substrate chinogona kukanganisa mashandiro eES-ERED. Zvisinei, kudziviswa uku kunogona kuderedzwa nekuwedzera substrate.
Muenzaniso 1(α,β-unsaturated aldehydes kana ketones)(1):
Muenzaniso 2(α,β-unsaturated carboxylic acids uye zvinobva mazviri)(2):
Chengeta kwemakore maviri pasi pe -20℃.
Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent yakakwira.
1. Lucídio C, Fardelone J, Augusto R, e tal. J.Mol.Catal.B:Enzym., 2004, 29: 41-45.
2. Stueckler C, Hall M, Ehammer H, etal. .Org.Lett, 2007, 9(26): 5409-5411.
