Hydroaminase (HAM)
Tsanangudzo Pfupi
Pamusoro peHydroaminase
Kune marudzi maviri echigadzirwa cheHAM enzyme (Nhamba seES-HAM-101~ ES-HAM-102) yakagadzirwa neSyncoZymes.HAM inogona kukonzeresa ammoniation yeanoic acid kana zvinobva pairi kugadzira chiral amino acids.HAM inogona kushandiswa kugadzira chiral amino acids (kana chiral amines) kubva kuenoic acids (kana alkenes).Ammonia donor, senge ammonia mvura kana ammonium munyu, inofanirwa.
Hydroaminase catalytic reaction type
Nharembozha/Wechat/WhatsApp: +86-13681683526
E-Maillchen@syncozymes.com
| Enzymes | Product Code | Tsanangudzo |
| Enzyme Powder | ES-HAM-101~ ES-HAM-102 | seti ye2 Nuclosidephosphoryalse, 50 mg imwe neimwe 2 zvinhu * 50mg / chinhu, kana humwe huwandu |
Iyo inotevera reaction system inogona kushandiswa kuratidza:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
10% (v/v): Amoniya
Gadzirisa pH kusvika 9.0 usati wawedzera enzyme, uye enzyme inofanira kuwedzerwa pakupedzisira.Isai maitikiro acho paanogara tembiricha shaker (semuenzaniso, 30oC, 150rpm) mukati kwemaawa makumi maviri nemana kusvika makumi mana nemasere.Centrifuge musanganiswa wega wega kuti uwedzere mapuroteni, iwo mashura anogona kushandiswa TLC, HPLC kana GC kuongororwa.
Cherechedza (1): Organic solvent (yakadai seDMSO, methanol, acetonitrile, IPA, nezvimwewo) inosarudzwa, inoshandiswa kune insoluble substrates.
