Hydroaminase (HAM)
Tsananguro pfupi:
Nezve Hydroaminase
Kune mhando mbiri dzechinhu chinogadzirwa neHAM enzyme (Nhamba seES-HAM-101~ ES-HAM-102) chakagadzirwa neSyncoZymes. HAM inogona kukonzera ammonia yeenoic acid kana zvinobva mairi kuti igadzire chiral amino acids. HAM inogona kushandiswa kugadzira chiral amino acids (kana chiral amines) kubva kuenoic acids (kana alkenes). Ammonia donor, senge ammonia water kana ammonium salt, inodiwa.
Hydroaminase catalytic reaction rudzi
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Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-HAM-101~ ES-HAM-102 | seti yeNuclosidephosphoryalse mbiri, 50 mg chimwe nechimwe zvinhu zviviri * 50mg / chinhu, kana chimwe chiyero |
Sisitimu inotevera ye reaction inogona kushandiswa sekutarisa:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Chinonyungudutsa cheorganic (1)
10% (v/v): Amoniya
Gadzirisa pH kusvika pa9.0 usati wawedzera enzyme, uye enzyme inofanira kuwedzerwa pakupedzisira. Isai reactions pachinodziya tembiricha (semuenzaniso, 30oC, 150rpm) kwemaawa 24 ~ 48. Shandisai centrifuge musanganiswa wega wega kuti muite mapuroteni, uye supernatant inogona kushandiswa pakuongorora kweTLC, HPLC kana GC.
Cherechedza (1): Chinhu chinonyungudutsa cheorganic (senge DMSO, methanol, acetonitrile, IPA, nezvimwewo) hachidiwi, chinoshandiswa pazvinhu zvisinganyunguduke.
