Nucloside phosphorylse (NP)

Tsananguro pfupi:

Nezve nucleoside phosphorylse

Kune mhando nhatu dzezvigadzirwa zve NP enzyme (Nhamba se ES-NP-101~ ES-NP-103) zvakagadzirwa neSyncoZymes. ES-NP-101 ipurine nucleoside phosphorylase, ES-NP-102 uye ES-NP-103 ipyrimidine nucleoside phosphorylase. Nucleosidephosphorylase inogona kuputsa nucleosides kuita mabhesi uye pentose phosphate. Nucleoside phosphorylase inogona kukamurwa kuita purine nucleoside phosphorylase uye pyrimidine nucleoside phosphorylase zvichienderana nezvinodiwa ne nucleoside bases. Purine nucleoside phosphorylase inogona kusanganisa adenosine ne adenine, inosine ne hypoxanthine, guanosine ne guanine, uye kugadzira ribose phosphate panguva imwe chete. Pyrimidine nucleoside phosphorylase inogona kusanganisa uridine ne uracil uye kugadzira ribose phosphate.

Nharembozha/Wechat/WhatsApp: +86-13681683526

Tsamba yemagetsi:lchen@syncozymes.com

Tumira email kwatiri

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Rudzi rwekuita kweNucloside phosphorylse:

Ruzivo rweChigadzirwa:

MaEnzayimu	Kodhi Yechigadzirwa	Tsanangudzo
Upfu hweEnzimu	ES-NP-101~ ES-NP-103	seti yeNuclosidephosphoryalse nhatu, 50 mg chimwe nechimwe zvinhu zvitatu * 50mg / chinhu, kana chimwe chiyero

Maitiro ekuongorora maitiro eNucloside phosphorylse:

Sisitimu inotevera ye reaction inogona kushandiswa sekutarisa:
Chikamu chepasi che 1-10 mg/ml
10 mg/ml Enzimu
100 mM Phosphate buffer (pH7.0)
Isai ma "reactions" muchishaker chekupisa chinogara chiripo (semuenzaniso, 30oC, 150rpm) kwemaawa 12-24. Isai musanganiswa wega wega pakati pe "centrifuge" kuti muwane mapuroteni, uye supernatant inogona kushandiswa pakuongorora kweTLC, HPLC kana GC.