Esterase neLipase (PLE neCALB)
Kune mhando makumi maviri nematanhatu dzezvigadzirwa zvePLE enzyme (Nhamba seES-PLE-101～ES-PLE-126) zvakagadzirwa neSyncoZymes. ES-PLE inogona kushandiswa pakunyungudutsa macompounds e aliphatic ne ester, kana regioselective ne stereoselective resolution kugadzira chiral acids ne derivatives dzadzo.
Rudzi rwekuita kweCatalytic:
★ Hunhu hwepamusoro hwe substrate.
★ Kusarudza kwakasimba kwerudo rwechikara.
★ Kuchinja kwakanyanya.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.
➢ Kazhinji, nzira yekugadzirira inofanira kusanganisira substrate, buffer solution uye ES-PLE. Kugadziriswa kwemamwe maES-PLE kunoitwa muchikamu cheorganic.
➢ Mhando dzese dzeES-PLE dzinoenderana nemamiriro akasiyana-siyana ekuita zvakanaka dzinofanira kudzidzwa imwe neimwe.
Muenzaniso 1 (Biosynthesis yePregabalin Intermediate)(1):
Muenzaniso 2(2):
Muenzaniso 3(3):
Muenzaniso 4(4):
Chengeta kwemakore maviri pasi pe -20℃.
Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera.
1. Xu FX, Chen SY, Xu G, etal. Appl. Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal. J. Am. Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal. Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917
