Esterase&Lipase (PLE&CALB)
Kune makumi maviri nenhanhatu emhando dzePLE enzyme zvigadzirwa (Nhamba seES-PLE-101～ES-PLE-126) yakagadzirwa neSyncoZymes.ES-PLE inogona kushandiswa kuita hydrolysis yealiphatic uye ester komisheni, kana regioselective uye stereoselective resolution yekugadzira chiral acids uye zvinobva kwavari.
Catalytic reaction type:
★ High substrate chaiyo.
★ Strong chiral selectivity.
★ Kutendeuka kwepamusoro.
★ Zvishoma ne-zvigadzirwa.
★ Mild reaction conditions.
★ Kuchengetedza zvakatipoteredza.
➢ Kazhinji, iyo reaction system inofanirwa kusanganisira substrate, buffer mhinduro uye ES-PLE.Iyo esterification yevamwe ES-PLE inoitwa muchikamu che organic.
➢ Mhando dzese dzeES-PLE dzinoenderana neakasiyana optimum reaction mamiriro anofanirwa kudzidzwa ega.
Muenzaniso 1(Biosynthesis yePregabalin Yepakati)(1):
Muenzaniso 2(2):
Muenzaniso 3(3):
Muenzaniso 4(4):
Chengeta makore maviri pasi -20 ℃.
Usambofa wakasangana nemamiriro akanyanya akadai se: tembiricha yepamusoro, yakakwirira / yakaderera pH.
1. Xu FX, Chen SY, Xu G, e tal.Appl.Biotechnol Bioproc E, 1988, 54(4): 1030.
2. Huang, FC, Lee, LF, Mittal, RSD e tal.J. Am.Chem.Soc, 1975, 97, 4144.
3. Kielbasinski, P., Goralczyk, P., Mikolajczyk, M., e tal.Synlett, 1994, 127.
4. Gais, HJ, Griebel, C., Buschmann, H. Tetrahedron:Asymmetry 2000, 11, 917
