Nitrilase (NIT)
Tsananguro pfupi:
NezveNitrilase
ES-NITs: Shandura zvakananga cyanide group kuita carboxyl group, kugadzira carboxylic acid, iyo inoshandiswa zvakanyanya mukurapa, kuchengetedza nharaunda, indasitiri yemakemikari, chinowedzera chikafu, machira, kugadzirwa kwekurima nedzimwe nzvimbo.
Kune mhando makumi mana dzezvigadzirwa zveNitrilase (NIT) (Nhamba seES-NIT-101~ES-NIT-140) zvakagadzirwa neSyncoZymes. ES-NIT chishandiso chinobatsira pakugadzira chiral carboxylic acids kuburikidza ne regio- uye stereoselective hydrolysis yemhando dzakasiyana dze aliphatic ne aromatic nitriles.
Rudzi rwekuita kweCatalytic:
Nharembozha/Wechat/WhatsApp: +86-13681683526
Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-NIT-101~ ES-NIT-140 | seti ye40 Nitrilase, 50 mg chimwe nechimwe 40 zvinhu * 50mg / chinhu, kana chimwe chiyero |
| Kiti Yekuongorora (SynKit) | ES-NIT-4000 | seti ye40 Nitrilase, 1 mg chimwe nechimwe zvinhu 40 * 1mg / chinhu |
★ Hunhu hwepamusoro hwe substrate.
★ Kuchinja kwakanyanya.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.
➢ Kazhinji, nzira yekugadzirira inofanira kusanganisira substrate, buffer solution (optimum reaction pH) uye ES-NIT.
➢ MaES-NIT ese anogona kuyedzwa zvakateerana mu reaction system iri pamusoro apa kana neNIT Screening Kit (SynKit NIT).
➢ Mhando dzese dzeES-NIT dzinoenderana nemamiriro akasiyana-siyana ekuita zvakanaka dzinofanira kudzidzwa imwe neimwe.
➢ Substrate yakawanda kana chigadzirwa chine substrate chinogona kukanganisa mashandiro eES-NIT. Zvisinei, kudziviswa uku kunogona kuderedzwa nekuwedzera substrate.
Muenzaniso 1 (Kugadzirwa kwe nicotinic acid)(1):
Muenzaniso 2 (Kugadzirwa kwe (R)-(-)-mandelic acid)(2):
Muenzaniso 3 (Kugadzirwa kwebaclofen)(3):
Muenzaniso 4 (Kugadzirwa kweglycolic acid)(4):
Chengeta kwemakore maviri pasi pe -20℃.
Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent yakakwira.
1 Mathew CD, Nagasawa T, Kobayashi M, etal. Appl. Environ. Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal. Appl. Environ. Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, uye tal. Chin. J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, uye tal. Biotechnol. Bioeng., 2008, 99(3): 717.
