Nitrilase (NIT)
Tsanangudzo Pfupi
About Nitrilase
ES-NITs: Kushandura zvakananga cyanide boka kuita carboxyl boka, kugadzirira carboxylic acid, iyo inoshandiswa zvakanyanya mukurapa, kuchengetedza kwezvakatipoteredza, indasitiri yemakemikari, kuwedzera kwechikafu, machira, kugadzirwa kwekurima uye mamwe minda.
Kune makumi mana emhando dzeNitrilase(NIT) zvigadzirwa (Nhamba seES-NIT-101~ES-NIT-140) yakagadzirwa neSyncoZymes.ES-NIT chishandiso chinobatsira kukonzeresa kuumbwa kwechiral carboxylic acids kuburikidza neregio- uye stereoelective hydrolysis yeakasiyana-siyana aliphatic uye anonhuwirira nitriles.
Catalytic reaction type:
Nharembozha/Wechat/WhatsApp: +86-13681683526
E-Maillchen@syncozymes.com
| Enzymes | Product Code | Tsanangudzo |
| Enzyme Powder | ES-NIT-101~ ES-NIT-140 | seti ye40 Nitrilase, 50 mg imwe neimwe 40 zvinhu * 50mg / chinhu, kana humwe huwandu |
| Screening Kit (SynKit) | ES-NIT-4000 | seti ye40 Nitrilase, 1 mg imwe neimwe 40 zvinhu * 1mg / chinhu |
★ High substrate chaiyo.
★ Kutendeuka kwepamusoro.
★ Zvishoma ne-zvigadzirwa.
★ Mild reaction conditions.
★ Kuchengetedza zvakatipoteredza.
➢ Kazhinji, maitiro ekuita anofanira kusanganisira substrate, buffer solution (optimum reaction pH) uye ES-NIT.
➢ Ese maES-NIT anogona kuyedzwa zvakateerana mumaitiro ari pamusoro kana neNIT Screening Kit (SynKit NIT).
➢ Ese marudzi eES-NIT anoenderana neakasiyana optimum reaction mamiriro anofanirwa kudzidzwa ega.
➢ Yakakwirira yekumisikidza Substrate kana chigadzirwa chine chinogona kutadzisa ES-NIT kuita.Nekudaro, iyo inhibition inogona kudzikiswa nebatch kuwedzera kwe substrate.
Muenzaniso 1(Synthesis ye nicotinic acid)(1):
Muenzaniso 2(Synthesis of (R)-(-)-mandelic acid)(2):
Muenzaniso 3(Synthesis of baclofen)(3):
Muenzaniso 4(Synthesis yeglycolic acid)(4):
Chengeta makore maviri pasi -20 ℃.
Usambofa wakasangana nemamiriro akanyanya akadai se: tembiricha yakakwira, yakakwira / yakaderera pH uye yakakwira concentration organic solvent.
1 Mathew CD, Nagasawa T, Kobayashi M, e tal.Appl.Environ.Microbiol, 1988, 54(4): 1030.
2 Yamamoto K, Oishi K, Fujimatsu I, e tal.Appl.Environ.Microbiol, 1991, 57(10): 3028.
3 Xu MZ, Ren J, Gong JS, uye tal.Chin.J. Biotechnol, 2013, 29(1): 31.
4 Wu S, Fogiel AJ, Petrillo KL, uye tal.Biotechnol.Bioeng., 2008, 99(3): 717.
