Benzaldehyde lyase (BAL)
Tsanangudzo Pfupi
Nezve Benzaldehyde lyase
ES-BAL (Benzaldehyde lyase): rudzi rwe enzyme inokonzeresa hydroxymethylation yeanonhuhwirira uye aliphatic aldehydes ine formaldehyde kugadzira inoenderana α- Hydroxymethyl ketone.BAL is thiamine diphosphate (ThDP) - inotsamira enzyme.Kune chete mhando imwe chete yeBAL enzyme chigadzirwa (Nhamba seES-BAL101) yakagadziriswa neSyncoZymes, inogona kushandiswa hydroxymethylation yeanonhuwirira uye aliphatic aldehydes.
Catalytic reaction type:
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★ High substrate chaiyo.
★ Strong chiral selectivity.
★ Kutendeuka kwepamusoro.
★ Zvishoma ne-zvigadzirwa.
★ Mild reaction conditions.
★ Kuchengetedza zvakatipoteredza.
➢ Kazhinji, maitiro ekuita anofanira kusanganisira substrate, buffer solution, enzyme, ThDP, Mg2+.
➢ BAL inofanirwa kuwedzerwa kwekupedzisira mukuita system, mushure mekunge pH uye tembiricha zvagadziriswa kune maitiro ekuita.
Muenzaniso 1(Synthesis yeN-inotsiviwa 1,2 amino alcohols kubva kualdehydes)(1):
Chengeta makore maviri pasi -20 ℃.
Usambofa wakasangana nemamiriro akanyanya akadai se: tembiricha yakakwira, yakakwira / yakaderera pH uye yakakwira concentration organic solvent.
1. Li Y, Hu N, Xu Z, nevamwe.Angewandte Chemie International Edition, 2022, 61(17): e202116344.
