Nitrile hydratase (NHT)
Tsananguro pfupi:
Nezve ES-NHTs
ES-NHTs: rudzi rwemetalloenzyme, iyo inonyungudutsa nitriles muamide yekutanga inoenderana nayo. Inoshandiswa zvakanyanya kwete chete pakugadzira amino acids, amides, carboxylic acids uye zvinobva mazviri, asiwo pakugadzira mishonga ye chiral. Kune mhando makumi maviri nemana dze nitrile hydratases dzakagadzirwa neSyncoZymes (Nhamba seES-NHT-101～ES-NHT-124).
Rudzi rwekuita kweCatalytic:
Nharembozha/Wechat/WhatsApp: +86-13681683526
Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-NHT-101~ ES-NHT-124 | seti yemaNitrile Hydratases makumi maviri nemana, 50 mg chimwe nechimwe, zvinhu makumi maviri nemana * 50mg / chinhu, kana chimwe chiyero |
| Kiti Yekuongorora (SynKit) | ES-NHT-2400 | seti yemaNnitrile Hydratases makumi maviri nemana, 1 mg chimwe nechimwe zvinhu makumi maviri nemana * 1mg / chinhu |
★ Hunhu hwepamusoro hwe substrate.
★ Kusarudza kwakasimba kwerudo rwechikara.
★ Kuchinja kwakanyanya.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.
➢ Kazhinji, nzira yekugadzirira inofanira kusanganisira substrate, buffer solution (optimum reaction pH) uye ES-NHT.
➢ MaES-NHT ese anogona kuyedzwa zvakateerana mu reaction system iri pamusoro apa kana neNHT Screening Kit (SynKit NHT).
➢ Mhando dzese dzeES-NHTs dzinoenderana nemamiriro akasiyana-siyana ekuita zvakanaka dzinofanira kudzidzwa imwe neimwe.
➢ Substrate yakawanda kana chigadzirwa chine chinogona kukanganisa mashandiro eES-NHT. Zvisinei, kudziviswa kunogona kuderedzwa nekuwedzera substrate, kubatana ne amidases kana kubviswa kwechigadzirwa.
Muenzaniso 1 (kubatana ne amidase)(1):
Muenzaniso 2(2):
Chengeta kwemakore maviri pasi pe -20℃.
Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent yakakwira.
1 Vojtech V, Ludmila M, Alicja BV, uye tal. J Mol Catal B-enzym, 2011, 71: 51-55.
2 Gary W. B, Thomas G, Christopher B. M, et tal. Tetrahedron lett, 2010, 51: 1639-1641.
