Nitrile reductase (NRED)
Tsanangudzo Pfupi
Nezve Nitrile reductase
Kune mhando nhatu dzeNRED enzyme chigadzirwa (Nhamba seES-NRED101~ ES-NRED103) yakagadzirwa neSyncoZymes.NRED inogona kukonzera kudzikiswa kweboka re nitrile kune amine.Mukuita catalysis, NADPH inodiwa semutakuri wehydrogen.
Nitrile reductasecatalytic reaction type
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E-Maillchen@syncozymes.com
| Enzymes | Product Code | Tsanangudzo |
| Tsanangudzo | ES- NRED -101~ ES- NRED -103 | seti ye3 Nitrile Reductase, 50 mg imwe neimwe 3 zvinhu * 50mg / chinhu, kana humwe huwandu |
Iyo inotevera reaction system inogona kushandiswa kuratidza:
1-10 mg / ml Substrate
10 mg / ml Enzyme
10% (v/v) Organic cosolvent (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg / ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Isai maitikiro acho paanogara tembiricha shaker (semuenzaniso, 30oC, 150rpm) mukati kwemaawa makumi maviri nemana kusvika makumi mana nemasere.Centrifuge musanganiswa wega wega kuti uwedzere mapuroteni, iwo mashura anogona kushandiswa TLC, HPLC kana GC ongororo.
Cherechedza (1): Organic solvent (yakadai seDMSO, methanol, acetonitrile, IPA, nezvimwewo) inosarudzwa, inoshandiswa kune insoluble substrates.
