Nitrile reductase (NRED)
Tsananguro pfupi:
Nezve Nitrile reductase
Kune mhando nhatu dzechigadzirwa cheNRED enzyme (Nhamba seES-NRED101~ ES-NRED103) chakagadzirwa neSyncoZymes. NRED inogona kukonzera kudzikira kweboka re nitrile kuita amine. Mukuita kwe catalysis, NADPH inodiwa semutakuri wehydrogen.
Nitrile reductasecatalytic reaction type
Nharembozha/Wechat/WhatsApp: +86-13681683526
Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Tsanangudzo | ES- NRED -101~ ES- NRED -103 | seti ye3 Nitrile Reductase, 50 mg chimwe nechimwe zvinhu zvitatu * 50mg / chinhu, kana chimwe chiyero |
Sisitimu inotevera ye reaction inogona kushandiswa sekutarisa:
Chikamu chepasi che 1-10 mg/ml
10 mg/ml Enzimu
10% (v/v) Chinonyungudutsa cheorganic (1)
100 mM Phosphate buffer (pH7.0)
0.2 mg/ml NADP+
10 mg/ml Glucose
2 mg/ml Glucose dehydrogenase (GDH)
Isai ma "reactions" muchishaker chekupisa chinogara chiripo (semuenzaniso, 30oC, 150rpm) kwemaawa 24 ~ 48. Isai musanganiswa wega wega pakati pe "centrifuge" kuti muwane mapuroteni, saka supernatant inogona kushandiswa pakuongorora kweTLC, HPLC kana GC.
Cherechedza (1): Chinhu chinonyungudutsa cheorganic (senge DMSO, methanol, acetonitrile, IPA, nezvimwewo) hachidiwi, chinoshandiswa pazvinhu zvisinganyunguduke.
