Phenylalanine dehydrogenase (PDH)
Tsananguro pfupi:
Nezve Phenylalanine dehydrogenase
ES-PDH: Rudzi rweoxidoreductase. Mhinduro yakanaka inogona kukonzera deamination yeL phenylalanine munyu kana paine NAD kuti igadzire phenylpyruvate munyu, uye reverse reaction inogona kukonzera kugadzirwa kweamino acid munyu unoenderana ne phenylpyruvate munyu. Syncozymes yakagadzira zvinhu zvisere zve phenylalanine dehydrogenase (yakaverengwa ES-PDH-101~ES-PDH-108). ES-PDH inogona kushandiswa kugadzira phenylalanine munyu kana amino acid munyu unoenderana ne phenylpyruvic acid munyu kana derivatives.
Rudzi rwekuita kweCatalytic:
Nharembozha/Wechat/WhatsApp: +86-13681683526
Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-PDH-101~ ES-PDH-108 | seti ye8 Nitrile Reductase, 50 mg chimwe nechimwe zvinhu 8 * 50mg / chinhu, kana chimwe chiyero |
★ Hunhu hwepamusoro hwe substrate.
★ Kusarudza kwakasimba kwerudo rwechikara.
★ Kuchinja kwakanyanya.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.
➢ Kazhinji, nzira yekugadzirira inofanira kusanganisira substrate, buffer solution, enzyme, coenzyme (NAD(H)), coenzyme regeneration system (semuenzaniso ammonium formate uye formate dehydrogenase).
➢ Rudzi rumwe nerumwe rweES-PDH runoenderana nemamiriro akasiyana-siyana ekuita zvakanaka, ayo anogona kudzidzwa ega ega kana zvichidikanwa.
➢ ES-PDH inofanira kuwedzerwa pakupedzisira mu reaction system kuti chiitiko chirambe chiripo.
Muenzaniso 1 (Kugadzirwa kwezvinhu zvinobva mu phenylalanine kubva mu phenylpyruvate derivatives)(1):
Chengeta kwemakore maviri pasi pe -20℃.
Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent yakakwira.
1. Francesca Paradisi, Stuart Collins, nevamwe. Journal of Biotechnology, 2007, 128, 408–411.
