Lactate Dehydrogenase (LDH)
Tsanangudzo Pfupi
Nezve Lactate dehydrogenase
Kune 1 mhando yeLDH enzyme chigadzirwa (Nhamba seES-LDH) yakagadziriswa neSyncoZymes.LDH inogona kukonzera kuderedzwa kwepyruvate kuL-lactic acid, kana kuderedza mamwe akafanana α-keto acids.Mukuita catalysis, NADH inodiwa sehydrogen transporter.
Lactate dehydrogenasecatalytic reaction type
Nharembozha/Wechat/WhatsApp: +86-13681683526
E-Maillchen@syncozymes.com
| Enzymes | Product Code | Tsanangudzo |
| Enzyme Powder | ES-LDH-101 | seti ye1 Monoamine Oxidase, 50 mg imwe neimwe 1 zvinhu * 50mg / chinhu, kana humwe huwandu |
Iyo inotevera reaction system inogona kushandiswa kuratidza:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Gadzira dehydrogenase (FDH)
Isai maitikiro acho paanogara tembiricha shaker (semuenzaniso, 30oC, 150rpm) mukati kwemaawa makumi maviri nemana kusvika makumi mana nemasere.Centrifuge musanganiswa wega wega kuti uwedzere mapuroteni, iwo iwo mashura anogona kushandiswa kuTLC, HPLC kana GC kuongororwa.
Cherechedza (1): Organic solvent (yakadai seDMSO, methanol, acetonitrile, IPA, nezvimwewo) inosarudzwa, inoshandiswa kune insoluble substrates.
