CALB Isina Kufamba

Tsananguro pfupi:

CALB

Recombinant Lipase B inobva kuCandida antarctica (CALB) inogadzirwa nekuvirisa mumvura nePichia pastoris yakagadziriswa majini.

CALB inogona kushandiswa muchikamu chemvura kana chikamu cheorganic catalytic esterification, esterosis, transesterification, ring opening polyester synthesis, aminolysis, hydrolysis yeamides, acylation yeamines uye addition reaction.

CALB ine chiral selectivity yakanyanya uye chinzvimbo selectivity, saka inogona kushandiswa zvakanyanya mukugadzira mafuta, chikafu, mishonga, cosmetic uye mamwe maindasitiri emakemikari.

Nharembozha/Wechat/WhatsApp: +86-13681683526

Tsamba yemagetsi:lchen@syncozymes.com

Tumira email kwatiri

Ruzivo rweChigadzirwa

Matagi eChigadzirwa

CALB Isina Kufamba:

CALB inodzivirirwa nekunyungudika kwemvura pa resin ine hydrophobic yakawanda iyo ine mapopoma macro, styrene/methacrylate polymer. CALB isina kushanduka yakakodzera kushandiswa mu organic solvents uye masisitimu asina solvent, uye inogona kudzokororwazve uye kushandiswazve kwenguva yakareba mumamiriro ezvinhu akakodzera.
Product Code: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Mabhenefiti eSZ-CALB-IMMO100:

★Kuita zvinhu zvakanyanya, kusarudza zviri nani uye kugadzikana kwakanyanya.
★Kushanda zvakanaka muzvikamu zvisina mvura.
★Bvisa zviri nyore kubva mu reaction system, bvisa reaction nekukurumidza, uye dzivisa mapuroteni akasara muchigadzirwa.
★ Inogona kudzokororwa uye kushandiswazve kuderedza mutengo.

Zvimiro zvechigadzirwa cheSZ-CALB-IMMO100:

Chiitiko	≥10000PLU/g
pH range ye reaction	5-9
Tembiricha yekusiyana kwekuita	10-60℃
Chitarisiko	CALB-IMMO100-A: Yero yakajeka kusvika kubrown yakasimba CALB-IMMO100-B: Chena kusvika kubrown yakapfava
Saizi yezvimedu	300-500μm
Kurasikirwa nekuomeswa pa105℃	0.5%-3.0%
Resin yekudzivirira kufamba	Polima ine maburi madiki, styrene/methacrylate
Reaction solvent	Mvura, organic solvent, nezvimwewo, kana isina solvent. Kuti pave nereaction mune zvimwe organic solvents, 3% yemvura inogona kuwedzerwa kuti ivandudze reaction effect
Saizi yezvimedu	CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm

Tsanangudzo yechikamu: Chikamu chimwe chinoenderana nekugadzirwa kwe1μmol per minute propyl laurate kubva ku lauric acid uye 1-propanol pa60℃. CALB-IMMP100-A neCALB-IMMO100-B zviri pamusoro apa zvinoenderana nekutakura zvinhu zvisina kufamba zvine saizi dzakasiyana dzezvikamu.

Mirayiridzo uye Zvekudzivirira:

1. Rudzi rwe reactor
Enzyme iyi isingafambi inoshanda kune kettle batch reactor pamwe ne fixed bed continuous flow reactor. Inofanira kucherechedzwa kuti isapwanyike nekuda kwesimba rekunze panguva yekudya kana kuzadza.
2. Kupindura pH, tembiricha uye solvent
Enzyme isina kufamba inofanira kuwedzerwa pakupedzisira, mushure mekunge zvimwe zvinhu zvawedzerwa uye zvanyungudutswa, uye pH inogadziriswa.
Kana kushandiswa kwesubstrate kana kuumbwa kwechigadzirwa kuchizokonzera shanduko yepH panguva yereaction, buffer yakakwana inofanira kuwedzerwa ku reaction system, kana kuti pH inofanira kutariswa uye kugadziriswa panguva yereaction.
Mukati meCALB inoshivirira tembiricha (pasi pe60 ℃), mwero wekushanduka wakawedzera nekuwedzera kwetembiricha. Pakushandisa, tembiricha yemhinduro inofanira kusarudzwa zvichienderana nekugadzikana kwesubstrate kana chigadzirwa.
Kazhinji, reaction ye ester hydrolysis yakakodzera mu aqueous phase system, nepo ester synthesis reaction yakakodzera mu organic phase system. Organic solvent inogona kuva ethanol, tetrahydrofuran, n-hexane, n-heptane uye toluene, kana mixed solvent yakakodzera. Kuti reaction iitike mune mamwe organic solvents, 3% yemvura inogona kuwedzerwa kuti ivandudze reaction effect.
3. Kushandisa zvakare uye hupenyu hweCALB
Kana paine chikonzero chakakodzera chekugadzirisa, CALB inogona kudzoserwa uye kushandiswazve, uye nguva chaiyo yekushandisa inosiyana nemapurojekiti akasiyana.
Kana CALB yakadzorerwa isingashandiswezve nguva dzose uye ichida kuchengetwa mushure mekudzorerwa, inofanira kugezwa, kuomeswa uye kuvharwa pa 2-8 ℃.
Mushure mekushandisazve kakawanda, kana kushanda kwe reaction kwadzikira zvishoma, CALB inogona kuwedzerwa zvakakodzera yoramba ichishandiswa. Kana kushanda kwe reaction kwadzikira zvakanyanya, inofanira kutsiviwa.

Mienzaniso yeMashandisirwo:

Muenzaniso 1 (Aminolysis)⁽¹⁾:

Muenzaniso 2 (Aminolysis)⁽²⁾:

Muenzaniso 3 (Kugadzira polyester inovhura ringi)⁽³⁾:

Muenzaniso 4 (Transesterification, regioselective yeboka rehydroxyl)⁽⁴⁾:

Muenzaniso 5 (Kuchinja maester, kugadziriswa kwekinetic kweracemic alcohols)⁽⁵⁾:

Muenzaniso 6 (Esterification, kinetic resolution ye carboxylic acid)⁽⁶⁾:

Muenzaniso 7 (Esterolysis, kinetic resolution)⁽⁷⁾:

Muenzaniso 8 (Hydrolysis ye amides)⁽⁸⁾:

Muenzaniso 9 (Kusanganiswa kwemamine)⁽⁹⁾:

Muenzaniso 10 (Aza-Michael addition reaction)⁽¹⁰⁾:

Mareferensi:

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