Immobilized CALB

Tsanangudzo Pfupi

CALB

Recombinant Lipase B kubva kuCandida antarctica (CALB) inogadzirwa nekuvirisa kwakanyudzwa mumvura ine genetically modified Pichia pastoris.

CALB inogona kushandiswa muchikamu chemvura kana organic phase catalytic esterification, esterolysis, transesterification, mhete yekuvhura polyester synthesis, aminolysis, hydrolysis yeamides, acylation yemamines uye kuwedzera maitiro.

CALB ine high chiral selectivity uye sarudzo yekusarudza, saka inogona kushandiswa zvakanyanya mukugadzira mafuta, chikafu, mishonga, cosmetic uye mamwe maindasitiri emakemikari.

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Immobilized CALB:

CALB haifambiswi nekushambadza kwemuviri pane yakanyanya hydrophobic resin iyo macroporous, styrene/methacrylate polymer.Immobilized CALB inokodzera maapplication ari muorganic solvents uye solvent-isina masisitimu, uye inogona kudzokororwa uye kushandiswazve kwenguva zhinji mumamiriro akakodzera.
Product Code: SZ-CALB- IMMO100A, SZ-CALB- IMMO100B.

Zvakanakira zveSZ-CALB-IMMO100:

★Kuitwa kwepamusoro, kusarudzwa kwepamusoro kwechiral uye kugadzikana kwepamusoro.
★Kuita zvirinani muzvikamu zvisiri zvemvura.
★Bvisa zviri nyore kubva mumaitiro ekuita, kurumidza kumisa kuita, uye dzivirira mapuroteni asara muchigadzirwa.
★Inogona kushandiswa zvakare uye kushandiswa zvakare kuderedza mutengo.

Zvigadzirwa Zvikamu zveSZ-CALB-IMMO100:

Chiitiko	≥10000PLU/g
pH renji rekuita	5-9
Tembiricha renji rekuita	10-60 ℃
Chitarisiko	CALB-IMMO100-A: Chiedza chero kusvika kubrown chakasimba CALB-IMMO100-B: Ichena kusvika kune yakasvibira
Chikamu saizi	300-500μm
Kurasika pakuomeswa pa105 ℃	0.5% -3.0%
Resin ye immobilization	Macroporous, styrene/methacrylate polymer
Reaction solvent	Mvura, organic solvent, nezvimwewo, kana isina solvent.Nekuita mune mamwe organic solvents, 3% mvura inogona kuwedzerwa kuti ivandudze maitiro ekuita
Chikamu saizi	CALB-IMMO100-A: 200-800 μm CALB-IMMO100-B: 400-1200 μm

Tsanangudzo yeyuniti: 1 unit inoenderana nekuumbwa kwe1μmol paminiti propyl laurate kubva kulauric acid uye 1-propanol pa60 ℃.Iyo iri pamusoro CALB-IMMP100-A uye CALB-IMMO100-B inoenderana neimmobilized vatakuri vane akasiyana particle size.

Mirayiridzo uye Chengetedzo:

1. Reactor mhando
Iyo immobilized enzyme inoshanda kune ese kettle batch reactor uye yakagadziriswa mubhedha inoenderera ichiyerera reactor.Izvo zvinofanirwa kucherechedzwa kudzivirira kupwanya nekuda kwesimba rekunze panguva yekudya kana kuzadza.
2. Reaction pH, tembiricha uye solvent
Iyo immobilized enzyme inofanirwa kuwedzerwa kwekupedzisira, mushure mezvimwe zvinhu zvawedzerwa nekunyungudutswa, uye pH yakagadziriswa.
Kana kushandiswa kwe substrate kana kuumbwa kwechigadzirwa kuchizotungamira kushanduko yepH panguva yekuita, buffer yakakwana inofanirwa kuwedzerwa kune reaction system, kana iyo pH inofanirwa kutariswa nekugadziriswa panguva yekuita.
Mukati mekushisa kwekushivirira kweCALB (pasi pe60 ℃), chiyero chekushandura chakawedzera nekuwedzera kwekushisa.Mukushandiswa kunoshanda, tembiricha yekupindura inofanirwa kusarudzwa zvinoenderana nekugadzikana kweiyo substrate kana chigadzirwa.
Kazhinji, iyo ester hydrolysis reaction inokodzera mune aqueous phase system, nepo ester synthesis reaction yakakodzera mune organic chikamu system.Iyo organic solvent inogona kuva ethanol, tetrahydrofuran, n-hexane, n-heptane uye toluene, kana yakakodzera yakasanganiswa solvent.Nekuita mune mamwe organic solvents, 3% mvura inogona kuwedzerwa kuti ivandudze maitiro ekuita.
3. Kushandisazve uye hupenyu hwebasa hweCALB
Pasi pemamiriro ekuita akakodzera, CALB inogona kudzoserwa uye kushandiswazve, uye yakatarwa nguva yekushandisa inosiyana nemapurojekiti akasiyana.
Kana iyo CALB yakadzoserwa ikasashandiswa zvekare uye ichida kuchengetwa mushure mekupora, inoda kugezwa nekuomeswa nekuvharwa pa2-8 ℃.
Mushure mekutenderera kwakati wandei kwekushandisazve, kana kuita kwekuita kwakaderedzwa zvishoma, CALB inogona kuwedzerwa nemazvo uye kuenderera mberi nekushandisa.Kana maitiro ekuita akaderedzwa zvakanyanya, inoda kutsiviwa.

Mienzaniso Yekushandisa:

Muenzaniso 1(Aminolysis)⁽¹⁾:

Muenzaniso 2(Aminolysis)⁽²⁾:

Muenzaniso 3(Ring inovhura polyester synthesis)⁽³⁾:

Muenzaniso 4(Transesterification, regioselective yeboka rehydroxyl)⁽⁴⁾:

Muenzaniso 5(Transesterification, kinetic resolution yeracemic alcohols)⁽⁵⁾:

Muenzaniso 6 (Esterification, kinetic kugadziriswa kwecarboxylic acid)⁽⁶⁾:

Muenzaniso 7(Esterolysis, kinetic resolution)⁽⁷⁾:

Muenzaniso 8(Hydrolysis yeamides)⁽⁸⁾:

Muenzaniso 9(Acylation yemamines)⁽⁹⁾:

Muenzaniso 10(Aza-Michael kuwedzera maitiro)⁽¹⁰⁾:

References:

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