Leucine Dehydrogenase (LeuDH)
Tsanangudzo Pfupi
Nezve Leucine dehydrogenase
Kune rudzi rumwe rweLeuDH enzyme chigadzirwa (Nhamba seES-LeuDH-101) yakagadzirwa neSyncoZymes.LeuDH inogona kukonzera reductive ammoniation ye4-methylbutanoic acid (munyu) kuL-leucine kana kuti oxidation ye leucine kune 4-methylbutanoic acid.ES-LeuDH inogonawo kukonzera reductive amination ye α-keto acids ine chimiro chakafanana kugadzirira zvinoenderana neamino acid derivatives.Mukuita catalysis, NADH inodiwa sehydrogen transporter.
Leucine dehydrogenasecatalytic reaction type
Nharembozha/Wechat/WhatsApp: +86-13681683526
E-Maillchen@syncozymes.com
| Enzymes | Product Code | Tsanangudzo |
| Enzyme Powder | ES-LeuDH-101 | seti ye1 Monoamine Oxidase, 50 mg imwe neimwe 1 zvinhu * 50mg / chinhu, kana humwe huwandu |
Iyo inotevera reaction system inogona kushandiswa kuratidza:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Organic cosolvent (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Gadzira dehydrogenase (FDH)
Isai maitikiro acho paanogara tembiricha shaker (semuenzaniso, 30oC, 150rpm) mukati kwemaawa makumi maviri nemana kusvika makumi mana nemasere.Centrifuge musanganiswa wega wega kuti uwedzere mapuroteni, iwo iwo mashura anogona kushandiswa kuTLC, HPLC kana GC kuongororwa.
Cherechedza (1): Organic solvent (yakadai seDMSO, methanol, acetonitrile, IPA, nezvimwewo) inosarudzwa, inoshandiswa kune insoluble substrates.
