Transaminase (ATA)
MaEnzme: Mapuroteni ndiwo mapuroteni anokonzera mamorekuru, maenzyme mazhinji ndiwo mapuroteni.
Transaminases: Rudzi rwema enzymes anokonzera amino kutamisa maamino acids nema keto acids. Transaminases ndiwo ma enzymes akakosha ehupenyu mukugadzirwa kwe asymmetric uye racemic resolution ye chiral amines.
Aminotransferase inogona kukamurwa kuita makirasi mana zvichienderana nekutevedzana uye chimiro: Ⅰ, Ⅱ, Ⅲ uye Ⅳ. ω-aminotransferases ndeye kirasi yechipiri transaminases, inowanzoshandiswa mukugadzira chiral amines uye amino acids zvisiri zvechisikigo, zvakaita se β-amino acids.
ω-aminotransferases: Kazhinji, ω-transaminase inoreva boka rema enzymes, ayo anokonzera kufambiswa kweammonia pasina α-amino acid se substrate kana chigadzirwa.
Maitiro ekugadzirisa:
| MaEnzayimu | Kodhi Yechigadzirwa | Kodhi Yechigadzirwa |
| Upfu hweEnzimu | ES-ATA-101~ ES-ATA-165 | seti ye65 ω-Transaminases, 50 mg chimwe nechimwe 65 zvinhu * 50mg / chinhu, kana chimwe chiyero |
| Kiti Yekuongorora (SynKit) | ES-ATA-6500 | seti ye65 ω-Transaminases, 1 mg chimwe nechimwe 65 zvinhu * 1mg / chinhu |
★ Hunhu hwepamusoro hwe substrate.
★ Kusarudza kwakasimba kwerudo rwechikara.
★ Kushanda zvakanyanya pakushandura.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.
➢ Kuongororwa kwema enzyme kunofanirwa kuitwa kune mamwe ma substrate nekuda kwehunhu hwe substrate, uye kuwana enzyme inokatyamadza substrate yacho nemhedzisiro yakanaka yekukatyamadza.
➢ Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent ine huwandu hwakawanda.
➢ Kazhinji, reaction system inofanira kusanganisira substrate, buffer solution, amino donor (senge amino acids uye 1-phenyl ethylamine) kana receptor (senge keto acids), coenzyme (PLP), cosolvent (senge DMSO).
➢ ATA inofanira kuwedzerwa pakupedzisira mu reaction system, mushure mekunge pH netembiricha zvagadziriswa kuti zvienderane nemamiriro e reaction.
➢ Mhando dzese dzeATA dzine mamiriro akasiyana-siyana ekuita zvakanaka, saka imwe neimwe inofanira kudzidzwa imwe neimwe.
Muenzaniso 1 (kugadzirwa kweSitagliptin, kugadzirwa kweasymmetric)(1):
Muenzaniso 2 (Mexiletine, Musanganiswa wekugadzirisa kinetic ne synthesis isina kuenzana)(2):
1 Savile CK, Janey JM, Mundorff EC, nevamwe. Science, 2010, 329(16), 305-309.
2 Koszelewski D, Pressnitz D, Clay D, nevamwe. Organic tsamba, 2009,11(21):4810-4812.
