Doro rinopisa (AOX)
Tsananguro pfupi:
Nezve alcohol oxidase
ES-AOXs: Ma enzyme aya anokwanisa kukonzera oxidation yemafuta alcohols kana aryl-alcohols kuti igadzire aldehydes. Anoshandisa molecular oxygen asi haadi cofactor yakawedzerwa kunze, uye hydrogen peroxide inogadzirwa.
Kune mhando nhanhatu dzezvigadzirwa zvealcohol oxidases (Nhamba seES-AOX-101~ES-AOX-106) zvakagadzirwa neSyncoZymes. ES-AOX101 neES-AOX102 dzinoda substrates dzealcohol dzakagadzirwa nealiphatic, ES-AOX103~ ES-AOX105 dzinoda substrates dzealcohol dzine aromatic, uye ES-AOX106 icholesterol oxidase. SZ-AOX chishandiso chinobatsira pakugadzira oxidation yemafuta ane alcohols kana aryl-alcohols kuti igadzire aldehydes.
Rudzi rwekuita kweCatalytic:
Nharembozha/Wechat/WhatsApp: +86-13681683526
Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-AOX-101~ ES-AOX-105 | seti yemaoxidases mashanu ealcohol, 50 mg chimwe nechimwe zvinhu zvishanu * 50mg / chinhu, kana chimwe chiyero |
| Kiti Yekuongorora (SynKit) | ES-AOX-500 | seti yemaoxidases mashanu ealcohol, 50 mg chimwe nechimwe zvinhu zvishanu * 50mg / chinhu, kana chimwe chiyero |
★ Hunhu hwepamusoro hwe substrate.
★ Kuchinja kwakanyanya.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.
➢ Kazhinji, nzira yekugadzirira inofanira kusanganisira substrate, buffer solution uye ES-AOX, uye oxygen inofanira kunge iripo.
➢ Mhando dzese dzeES-AOXs dzinoenderana nemamiriro akasiyana-siyana ekuita zvakanaka, ayo anogona kudzidzwa ega ega.
➢ Substrate yakawanda kana chigadzirwa chine substrate chinogona kukanganisa mashandiro eES-AOX. Zvisinei, kudziviswa kunogona kuderedzwa nekuwedzera substrate.
➢ Kuunganidzwa kweH2O2Muhurongwa huchaita kuti ma enzymes asashande, izvo zvinogona kugadziriswa zvinobudirira nekushandisa catalase.
Muenzaniso 1 (Kuoxidation ye aryl-alcohols)(1):
Muenzaniso 2 (Kuoxidation yemafuta ane alcohols)(2):
Chengeta kwemakore maviri pasi pe -20℃.
Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent yakakwira.
1. Benen J AE, Sa'nchez-Torres P, Wagemaker M JM, e tal. J Biol Chem, 1998, 273(14): 7865-7872.
2. Mauersberger S, Drechsler H, Oehme G, e tal. Appl Microbiol Biot, 1992, 37: 66-73.
