Ketoreductase (KRED)

Tsananguro pfupi:

Nezve Ketoreductase

MaEnzme: Mapuroteni ndiwo mapuroteni anokonzera mamorekuru, maenzyme mazhinji ndiwo mapuroteni.

Ketoreductase: Inonziwo "Carbonyl reductase", ma enzymes anokonzera reversible redox reaction pakati pealdehydes / ketones uye alcohols, uye anoda coenzymes seH transmitter senge NADH kana NADPH. Kune mapoka matatu makuru eKRED: Short-chain Dehydrogenase / Reductases (SDRs), Medium-chain Dehydrogenases / Reductases (MDRs), uye Aldo-KetoReductases (AKRs). Mabasa e catalytic evatatu ava akafanana, asi chimiro uye hunhu hwakasiyana zvikuru.

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Tsamba yemagetsi:lchen@syncozymes.com

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Ruzivo rweChigadzirwa

Matagi eChigadzirwa

Nezve Ketoreductase:

KRED kubva kuSyncoZymes: Kune mhando 191 dzezvigadzirwa zveKRED enzyme (Nhamba seES-KRED-101~ES-KRED-291) zvakagadzirwa neSyncoZymes. SZ-KRED inogona kushandiswa zvakanyanya mukuderedza maaldehydes, beta-ketone esters, α-ketone esters nemaketones munzvimbo dzakasiyana-siyana uye munzvimbo dzakasiyana.

Rudzi rwekuita kweCatalytic:

Ruzivo rweChigadzirwa:

MaEnzayimu	Kodhi Yechigadzirwa	Tsanangudzo
Upfu hweEnzimu	Upfu hweEnzimu	seti ye187 Ketoreductases, 50 mg chimwe nechimwe 187 zvinhu * 50mg / chinhu, kana chimwe chiyero
Kiti Yekuongorora (SynKit)	ES-KRED-18100	seti ye181 Ketoreductases, 1 mg chimwe nechimwe 181 zvinhu * 1mg / chinhu

Zvakanakira:

★ Hunhu hwepamusoro hwe substrate.
★ Kusarudza kwakasimba kwerudo rwechikara.
★ Kushanda zvakanyanya pakushandura.
★ Zvibereko zvishoma.
★ Mamiriro ekuita zvinhu zvisina kusimba.
★ Inochengetedza zvakatipoteredza.

Mirayiridzo yekushandisa:

➢ Kuongororwa kwema enzyme kunofanirwa kuitwa kune mamwe ma substrate nekuda kwehunhu hwe substrate, uye kuwana enzyme inokatyamadza substrate yacho nemhedzisiro yakanaka yekukatyamadza.
➢ Usambosangana nemamiriro ezvinhu akaipisisa akadai se: tembiricha yakakwira, pH yakakwira/yakaderera uye organic solvent ine huwandu hwakawanda.
➢ Kazhinji, nzira yekugadzirira inofanira kusanganisira substrate, buffer solution, coenzymes (NAD(H) kana NADP(H)), coenzyme regeneration system (semuenzaniso glucose neglucose dehydrogenase).
➢ KRED inofanira kuwedzerwa pakupedzisira mu reaction system, mushure mekunge pH netembiricha zvagadziriswa kuti zvienderane nemamiriro e reaction.
➢ Mhando dzese dzeKRED dzine mamiriro akasiyana-siyana ekuita zvakanaka, saka imwe neimwe inofanira kudzidzwa imwe neimwe.