Lactate Dehydrogenase (LDH)
Tsananguro pfupi:
Nezve Lactate dehydrogenase
Kune mhando imwe chete yechinhu chinogadzirwa neenzyme yeLDH (Nhamba seES-LDH) yakagadzirwa neSyncoZymes. LDH inogona kukonzera kudzikira kwepyruvate kuita L-lactic acid, kana kudzikira kwemamwe ma α-keto acids akafanana. Mukuita kwe catalysis, NADH inodiwa semutakuri wehydrogen.
Lactate dehydrogenasecatalytic reaction mhando
Nharembozha/Wechat/WhatsApp: +86-13681683526
Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-LDH-101 | seti yeMonoamine Oxidase imwe, 50 mg chimwe nechimwe 1 chinhu * 50mg / chinhu, kana chimwe chiyero |
Sisitimu inotevera ye reaction inogona kushandiswa sekutarisa:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Chinonyungudutsa cheorganic (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Isai ma "reactions" muchishaker chekupisa chinogara chiripo (semuenzaniso, 30oC, 150rpm) kwemaawa 24 ~ 48. Isai musanganiswa wega wega pakati pe "centrifuge" kuti muwane mapuroteni, saka supernatant inogona kushandiswa pakuongorora TLC, HPLC kana GC.
Cherechedza (1): Chinhu chinonyungudutsa cheorganic (senge DMSO, methanol, acetonitrile, IPA, nezvimwewo) hachidiwi, chinoshandiswa pazvinhu zvisinganyunguduke.
