Leucine Dehydrogenase (LeuDH)
Tsananguro pfupi:
Nezve Leucine dehydrogenase
Kune rudzi rumwe chete rweLeuDH enzyme product (Nhamba seES-LeuDH-101) yakagadzirwa neSyncoZymes. LeuDH inogona kukonzera ammoniation ye4-methylbutanoic acid (munyu) kuL-leucine kana oxidation yeleucine ku4-methylbutanoic acid. ES-LeuDH inogona zvakare kukonzera kuderedza kuderedza kweα-keto acids ine chimiro chakafanana kugadzira amino acid derivatives inoenderana. Mukuita kwe catalysis, NADH inodiwa semutakuri wehydrogen.
Leucine dehydrogenasecatalytic reaction rudzi
Nharembozha/Wechat/WhatsApp: +86-13681683526
Tsamba yemagetsi:lchen@syncozymes.com
| MaEnzayimu | Kodhi Yechigadzirwa | Tsanangudzo |
| Upfu hweEnzimu | ES-LeuDH-101 | seti yeMonoamine Oxidase imwe, 50 mg chimwe nechimwe 1 chinhu * 50mg / chinhu, kana chimwe chiyero |
Sisitimu inotevera ye reaction inogona kushandiswa sekutarisa:
1-10 mg/ml: Substrate
10 mg/ml: Enzyme
10% (v/v): Chinonyungudutsa cheorganic (1)
100 mM: Phosphate buffer (pH7.0)
0.2 mg/ml: NAD+
100 mM: Ammonium formate
10 mg/ml: Formate dehydrogenase (FDH)
Isai ma "reactions" muchishaker chekupisa chinogara chiripo (semuenzaniso, 30oC, 150rpm) kwemaawa 24 ~ 48. Isai musanganiswa wega wega pakati pe "centrifuge" kuti muwane mapuroteni, saka supernatant inogona kushandiswa pakuongorora TLC, HPLC kana GC.
Cherechedza (1): Chinhu chinonyungudutsa cheorganic (senge DMSO, methanol, acetonitrile, IPA, nezvimwewo) hachidiwi, chinoshandiswa pazvinhu zvisinganyunguduke.
